Species of Ramalina, Dirinaria, and Lecanora at El Maviri Beach: A First Report
Species of Ramalina, Dirinaria, and Lecanora at El Maviri Beach: A First Report
DOI:
https://doi.org/10.63622/RBS.2401Keywords:
Lichen, molecular identification, Ramalina, Dirinaria, LecanoraAbstract
Lichenized fungi are a part of lichen symbiotic associations, in which a fungus and a microalgae or cyanobacteria form a mutually beneficial relationship. This relationship allows lichenized fungi to thrive in a wide range of environments, including extreme conditions such as deserts and high altitudes. Due to their sensibility to atmospheric pollutants and their ability to absorb and retain metallic elements, they have been used as bioindicators of the ecosistem health. Despite their ecological significance, little is known about the species diversity of lichenized fungi in certain regions of México, such as the state of Sinaloa. In this study, a directed sampling was performed in “El Maviri” beach, located in the municipality of Ahome, Sinaloa, in which five lichen morphotypes were obtained. For their identification, their DNA was extracted through the CTAB method, the ITS region was amplified and a phylogenetic analysis was performed using the Maximum Likelihood method. In the analysis, three samples were identified as Ramalina sp., where two of them formed a sister clade with R. complanata and one with R. stenospora. On the other hand, one sample was identified as Dirinaria consimilis, while the last sample was identified as Lecanora sp., which formed a sister clade with L. argopholis. These findings emphasize the importance of “El Maviri” beach as a habitat for lichen diversity. Further studies should be conducted to explore the potential ecological roles of these lichen species and to assess their potential for medicinal or other practical uses.
References
Arup U, Holien H, Coppins BJ. 2023. Lecanora caledonica – a new species in the Lecanora intumescens group (Lecanoraceae) from north-western Europe. The Lichenologist, 55(3–4), 107–114. DOI: 10.1017/s0024282923000233
Asplund J, Wardle DA. 2016. How lichens impact terrestrial communities and ecosystem properties. Biological Reviews, 92(3), 1720-1738. DOI: 10.1111/brv.12305
Balaji P, Hariharan GN. 2013. Diversity of Macrolichens in Bolampatti II Forest Range (Siruvani Hills), Western Ghats, Tamil Nadu, India. ISRN Biodiversity, 2013, 1–7. DOI: 10.1155/2013/124020
Benítez N, Medina J, Vásquez C, Loaiza T, Luzuriaga Y, Calva J. 2019. Lichens and Bromeliads as Bioindicators of Heavy Metal Deposition in Ecuador. Diversity, 11(2), 28. DOI: 10.3390/d11020028
Bergamini A, Scheidegger C, Stofer S, et al. 2005. Performance of Macrolichens and Lichen Genera as Indicators of Lichen Species Richness and Composition. Conservation Biology, 19(4), 1051–1062. DOI: 10.1111/j.1523-1739.2005.00192.x-i1
Borchhardt N, Schiefelbein U, Abarca N, Boy J, Mikhailyuk T, Sipman HJ, Karsten U. 2017. Diversity of algae and lichens in biological soil crusts of Ardley and King George islands, Antarctica. Antarctic Science, 29(3), 229–237. DOI: 10.1017/s0954102016000638
Bowler PA, Rundel PW. 1972. A New Species of Ramalina from Sinaloa, Mexico. The Bryologist, 75(4), 574–576. DOI: 10.2307/3241213
Bowler PA, Rundel PW. 1973. Two New Lichens (Ramalina) from Baja California, Mexico. The Bryologist, 76(1), 211–213. DOI: 10.2307/3241251
Cardinale M, Vieira de Castro J, Müller H, Berg G, Grube M. 2008. In situ analysis of the bacterial community associated with the reindeer lichen Cladonia arbuscula reveals predominance of Alphaproteobacteria. FEMS Microbiology Ecology, 66(1), 63–71. DOI: 10.1111/j.1574-6941.2008.00546.x
Córdova-Chávez O, Castillo-Campos G, Pérez-Pérez RE, García-Franco JG, da Silva-Cáceres ME. 2016. Alpha Diversity of Lichens Associated with Quercus laurina in a Mountain Cloud Forest at Cofre de Perote Eastern Slope (La Cortadura), Veracruz, Mexico. Cryptogamie, Mycologie, 37(2), 193-204. DOI: 10.7872/crym/v37.iss2.2016.193
Culberson WL. 2002. Lichen Flora of the Greater Sonoran Desert Region—Volume 1. The Bryologist, 105(4), 725–725. DOI: 10.1639/0007-2745(2002)105[0725:lfotgs]2.0.co;2
Dos Santos LA, Aptroot A, Lücking R, Cáceres MES. 2023. Lecanora s.lat. (Ascomycota, Lecanoraceae) in Brazil: DNA Barcoding Coupled with Phenotype Characters Reveals Numerous Novel Species. Journal of Fungi, 9, 415. DOI: 10.3390/jof9040415
Doyle J. 1991. DNA Protocols for Plants. Molecular Techniques in Taxonomy, 283–293. DOI: 10.1007/978-3-642-83962-7_18
Edgar RC. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32(5), 1792–1797. DOI: 10.1093/nar/gkh340
Elkhateeb WA, Daba GM, Sheir D, Nguyen TD, Hapuarachchi, KK, Thomas PW. 2021. Mysterious World of Lichens: Highlights on Their History, Applications, and Pharmaceutical Potentials. The Natural Products Journal, 11(3), 275–287. DOI: 10.2174/2210315510666200128123237
Ellis CJ, Asplund J, Benesperi R, et al. 2021. Functional Traits in Lichen Ecology: A Review of Challenge and Opportunity. Microorganisms, 9(4), 766. DOI: 10.3390/microorganisms9040766
Etayo J, Sancho LG, Pino-Bodas R. 2023. Taxonomic and phylogenetic approach to some Antarctic lichenicolous fungi. Mycological Progress, 22(2). DOI: 10.1007/s11557-022-01860-7
Galloway DJ. 1992. Biodiversity: a lichenological perspective. Biodiversity and Conservation, 1(4), 312–323. DOI: 10.1007/bf00693767
Gerlach ADCL, Toprak Z, Naciri Y, Caviró EA, da Silveira, RMB, Clerc P. 2019. New insights into the Usnea cornuta aggregate (Parmeliaceae, lichenized Ascomycota): Molecular analysis reveals high genetic diversity correlated with chemistry. Molecular Phylogenetics and Evolution, 131, 125–137. DOI: 10.1016/j.ympev.2018.10.035
Grimm M, Grube M, Schiefelbein U, Zühlke D, Bernhardt J, Riedel K. 2021. The Lichens’ Microbiota, Still a Mystery? Frontiers in Microbiology, 12. DOI: 10.3389/fmicb.2021.623839
Grube M. 2010. Die Hard: Lichens. In: Seckbach J and Grube M (eds), Symbioses and Stress. Joint Ventures in Biology Vol. XVII: Springer, pp. 509–523. DOI: 10.1007/978-90-481-9449-0_26
Guzmán G, Pérez-Pérez RE, Ramírez-Juárez JA. 2021. La importancia de un jardín doméstico en la conservación de macrolíquenes cortícolas en Veracruz, México. Madera y bosques, 27(1), 1-10. DOI: 10.21829/myb.2021.2712068
Hall TA. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.
Hawksworth DL. 1988. The variety of fungal-algal symbioses, their evolutionary significance, and the nature of lichens. Botanical Journal of the Linnean Society, 96(1), 3–20. DOI: 10.1111/j.1095-8339.1988.tb00623.x
Hawksworth DL, Grube M. 2020. Lichens redefined as complex ecosystems. New Phytologist, 227(5), 1281–1283. DOI: 10.1111/nph.16630
Hawksworth DL, Lücking R. 2017. Fungal Diversity Revisited: 2.2 to 3.8 Million Species. Microbiology Spectrum, 5(4). DOI: 10.1128/microbiolspec.funk-0052-2016
Hayward GC, Blanchon DJ, Lumbsch HT. 2014. Molecular data support Ramalina ovalis as a distinct lineage (Ramalinaceae, Ascomycota). The Lichenologist, 46(4), 553–561. DOI: 10.1017/s0024282913000947
Herrera-Campos MDLN, Lücking R, Pérez-Pérez RE, Miranda-González R, Sánchez N, Barcenas-Peña A, Carrizosa A, Zambrano A, Ryan BD, Nash TH. 2014. Biodiversidad de líquenes en México. Revista Mexicana De Biodiversidad, 85, 82–99. DOI: 10.7550/rmb.37003
Ivanovich C, Dolnik C, Otte V, Palice Z, Sohrabi M, Printzen C. 2021. A preliminary phylogeny of the Lecanora saligna-group, with notes on species delimitation. The Lichenologist, 53, 63-79. DOI: 10.1017/S0024282921000074
Kashiwadani H, Nash TH. 2002. New species of the genus Ramalina (Ascomycotina: Ramalinaceae) from the sonoran desert, Mexico. Mycotaxon, 83, 385-389.
Kistenich S, Timdal E, Bendiksby M., Ekman S. 2018. Molecular systematics and character evolution in the lichen family Ramalinaceae (Ascomycota: Lecanorales). Taxon, 67(5), 871-904. DOI: 10.12705/675.1
Knudsen K, Lendemer JC, Schultz M, Kocourková J, Sheard JW, Pigniolo A, Wheeler T. 2017. Lichen biodiversity and ecology in the San Bernardino and San Jacinto Mountains in southern California (EUA). Opuscula Philolichenum, 16, 15-138.
LaGreca S, Lumbsch HT, Kukwa M, Wei X, Han JE, Moon KH, Kashiwadani H, Aptroot A, Leavitt SD. 2020. A molecular phylogenetic evaluation of the Ramalina siliquosa complex, with notes on species circumscription and relationships within Ramalina. The Lichenologist, 52(3), 197–211. DOI: 10.1017/S0024282920000110
Lawrey JD, Diederich P. 2003. Lichenicolous Fungi: Interactions, Evolution, and Biodiversity. The Bryologist, 106(1), 80–120. DOI: 10.1639/0007-2745(2003)106[0080:lfieab]2.0.co;2
Lee BG, Hur JS. 2020. A new lichenized fungus, Lecanora baekdudaeganensis, from Corea del sur, with a taxonomic key for Corean Lecanora species. MycoKeys, 70, 39–58. DOI: 10.3897/mycokeys.70.51569
León-González D, Pérez-Pérez RE. 2020. Líquenes epífitos en Juniperus flaccida Schltdl. (Cupressaceae) - componente importante de los bosques templados de Oaxaca, México. Acta Biológica Colombiana, 25(2), 235-245. DOI: 10.15446/abc.v25n2.77238
Li L, Zhang Y, Printzen C. 2023. Phylogeny, morphology and chemistry reveal two new multispored species in the Lecanora subfusca group (Lecanoraceae, Ascomycota). MycoKeys, 99, 25–43. DOI: 10.3897/mycokeys.99.108462
Lücking R. 1999. Folicolous lichens and their lichenicolous fungi from Ecuador, with a comparison of lowland and montane rain forest. Willdenowia, 29(1–2), 299–335. DOI: 10.3372/wi.29.2924
Magyar, D., Vass, M., Li, D. (2016). Dispersal strategies of microfungi. Fungal biology (pp. 315–371). https://doi.org/10.1007/978-3-319-29137-6_14
Martin KJ, Rygiewicz PT. 2005. Fungal-specific PCR primers developed for analysis of the ITS region of environmental DNA extracts. BMC Microbiol, 5, 28. DOI: 10.1186/1471-2180-5-28
McCune B. 2018. Biodiversity and ecology of lichens of Katmai and Lake Clark National Parks and Preserves, Alaska. Mycosphere, 9(4), 859–930. DOI: 10.5943/mycosphere/9/4/10
Miguel-Vázquez MI, Simijaca D, Pérez-Pérez RE, Ocampo G. 2021. Lichenized fungi of the arid zones of central Mexico: new records for the country and the state of Aguascalientes. Sydowia, 74, 15-31. DOI: 10.12905/0380.sydowia74-2021-0015
Miranda-González R, McCune B, Moldenke AR. 2023. Lichens as material for Lepidoptera's housing: A molecular approach to a widespread and highly selective interaction. Fungal Ecology, 61. DOI: 10.1016/j.funeco.2022.101195
Morillas L, Roales J, Cruz C, Munzi S. 2022. Lichen as Multipartner Symbiotic Relationships. Encyclopedia, 2(3), 1421–1431. DOI: 10.3390/encyclopedia2030096
Moya P, Molins A, Martínez-Alberola F, Muggia L, Barreno E. 2017. Unexpected associated microalgal diversity in the lichen Ramalina farinacea is uncovered by pyrosequencing analyses. PLOS ONE, 12(4), e0175091. DOI: 10.1371/journal.pone.0175091
Muggia L, Grube M. 2018. Fungal Diversity in Lichens: From Extremotolerance to Interactions with Algae. Life, 8(2), 15. DOI: 10.3390/life8020015
Nash TH, Ryan BD, Gries C, Bungartz F. 2004. Lichen Flora of the Greater Sonoran Desert Region. Vol 2. Lichen Unlimited: Tempe, AZ, Arizona State University.
Nash TH, Hertel H. 1997. Lecanora comoduensis (Lecanorales: Lecanoraceae), a New Species of Lichenized Fungi from the Sonoran Desert Region. The Bryologist, 100(3), 377–379. DOI: 10.2307/3244508
Park JS, Han SK, Oh SO. 2023. Lecanora neobarkmaniana (Lecanorales, Lecanoraceae), A New Lichen Species from Corea del sur. Mycobiology, 51(1), 16–25. DOI: 10.1080/12298093.2023.2168349
Pérez-Ortega S, Spribille T, Palice Z, Elix JA, Printzen C. 2010. A molecular phylogeny of the Lecanora varia group, including a new species from western North America. Mycological Progress, 9(4), 523–535. DOI: 10.1007/s11557-010-0660-y
Pérez-Pérez RE, Silva-Espejo R, Figueroa-Castro DM, Castañeda-Posadas C. 2024. Riqueza y composición de líquenes de los pueblos mágicos de Cuetzalan y Tlatlauquitepec, Puebla, México. Polibotánica, 58, 31-47. DOI: 10.18387/polibotanica.58.3
Ponce de León R. 1909. Ligeros apuntes sobre la flora del Estado de Sinaloa. Talleres Tipográficos de Julio G. Arce “Rosales” 24.
Poncet R, Lohezic-Le Dévéhat F, Ferron S, Hivert J, Fontaine C, Picot F, Bidault E, Kervran L. 2021. The genus Ramalina Ach. (Ascomycota, Lecanoromycetes, Ramalinaceae) from the Scattered Islands (French Southern and Antarctic Lands), with description of three new species. Plant and Fungal Systematics, 66 (2), 211-224.
Puy-Alquiza MJ, Gómez-Peralta M, Reyes-Zamudio V, Gregorio-Cipriano MR, Miranda-Avilés R, Rios-Ureña DN, Cortés-Hernández V. 2018. Diversidad de macrolíquenes saxícolas en México: caso de estudio del distrito minero de Guanajuato. Acta botánica mexicana, 123. DOI: 10.21829/abm123.2018.1246
Räisänen R. 2023. Natural Colorants from Lichens and Mushrooms. In: Stevens C, Bechtold T, Manian A and Pham T (eds). Handbook of Natural Colorants Vol. II, John Wiley & Sons, pp. 317–331. DOI: 10.1002/9781119811749.ch14
Raja HA, Miller AN, Pearce CJ, Oberlies NH. 2017. Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community. Journal of Natural Products, 80(3), 756–770. DOI: 10.1021/acs.jnatprod.6b01085
Rangsiruji A, Meesim S, Buaruang K, Boonpragob K, Mongkolsuk P, Binchai S, Pringsulaka O, Parnmen S. 2020. Molecular systematics and species distribution of foliose lichens in the gulf of thailand mangroves with emphasis on Dirinaria picta species complex. Songklanakarin Journal of Science & Technology, 42(3). 504-514.
Sánchez-Girón X, Cerros-Tlatilpa R, Pérez-Pérez RE. 2023. Diversidad de líquenes epífitos en bosques de Quercus de Morelos, México. Revista Bosque, 44(3), 581-594. DOI: 10.4067/S0717-92002023000300581
Schoch CL, Seifert KA, Huhndorf S et al. 2012. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the National Academy of Sciences, 109(16), 6241–6246. DOI: 10.1073/pnas.1117018109
Shah AA, Badshah L, Muhammad M, Basit A, Ullah I, Mohamed HI, Khan A. 2024. Secondary metabolites of lichens and their application. Fungal Secondary Metabolites, 91–115. DOI: 10.1016/b978-0-323-95241-5.00013-7
Shukla V, Upreti DK, Bajpai R. 2013. Lichen Diversity in Different Lichenogeographical Regions of India. Lichens to Biomonitor the Environment, 61–96. DOI: 10.1007/978-81-322-1503-5_4
Sipman HJM, Wolf JHD. 1998. Provisional checklist for the lichens of Chiapas. Acta Botanica Mexicana, 45, 1-29.
Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E. 2020. The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys, 73, 1–68. DOI: 10.3897/mycokeys.73.47287
Tekpinar AD, Kalmer A. 2019. Utility of various molecular markers in fungal identification and phylogeny. Nova Hedwigia, 109(1–2), 187–224. DOI: 10.1127/nova_hedwigia/2019/0528
Thakur M, Shrikhandia SP, Kumar V. 2022. A Lichens-Mediated Mechanism for Environmental Biodeterioration. Air, Soil and Water Research, 15, 1-11. DOI: 10.1177/11786221221131004
Zachariah SA, Varghese SK. 2018. The Lichen Symbiosis: A Review. International Journal of Scientific Research and Review, 7(3), 1160-11169.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Revista Bioc Scientia
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
